6XGM

Self-assembly of a 3D DNA crystal lattice (4x5 junction version) containing the J25 immobile Holliday junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	69.023, 69.023, 59.453
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.110
R-factor	0.2199
Rwork	0.219
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6x8c
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.780	50.000	3.150
High resolution limit [Å]	3.100	8.400	3.100
Rmerge	0.240	0.230	1.151
Rmeas	0.254	0.243	1.243
Rpim	0.081	0.077	0.459
Number of reflections	5321	290	190
<I/σ(I)>	5.1
Completeness [%]	93.1	100	65.7
Redundancy	8.4	10.5	5
CC(1/2)		0.965	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.5 mL of 0.05 M TRIS pH 8.0 with 10 mM MgCl2, 1.0 mM CoH18N6, and 20% Ethanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock