6XG8
ISCth4 transposase, pre-cleaved complex, PCC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2017-07-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.680, 109.500, 157.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.610 - 3.500 |
| R-factor | 0.2222 |
| Rwork | 0.221 |
| R-free | 0.26710 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.673 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.610 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.072 | 0.990 |
| Rmeas | 0.078 | |
| Number of reflections | 20108 | 1474 |
| <I/σ(I)> | 13.2 | 1.8 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 7.17 | 7.51 |
| CC(1/2) | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | HEPES, sodium chloride, sodium acetate, PEG4000 |
