6XG7

1.3 A Resolution Structure of the of the NHL Repeat Region of D. melanogaster Thin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	42.633, 51.917, 105.119
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.110 - 1.300
R-factor	0.14
Rwork	0.138
R-free	0.17400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d69
RMSD bond length	0.008
RMSD bond angle	1.004
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (dev_3581)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.550	46.550	1.320
High resolution limit [Å]	1.300	7.120	1.300
Rmerge	0.083	0.054	0.991
Total number of observations	376145	2429	18706
Number of reflections	58203	432	2836
<I/σ(I)>	10.7	25.9	1.8
Completeness [%]	99.9	99.7	99.9
Redundancy	6.5	5.6	6.6
CC(1/2)	0.998	0.997	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	25% (w/v) PEG 3350, 100 mM Bis-Tris, 200 mM ammonium sulfate