6XG7
1.3 A Resolution Structure of the of the NHL Repeat Region of D. melanogaster Thin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.633, 51.917, 105.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.110 - 1.300 |
| R-factor | 0.14 |
| Rwork | 0.138 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6d69 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.004 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (dev_3581) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.550 | 46.550 | 1.320 |
| High resolution limit [Å] | 1.300 | 7.120 | 1.300 |
| Rmerge | 0.083 | 0.054 | 0.991 |
| Total number of observations | 376145 | 2429 | 18706 |
| Number of reflections | 58203 | 432 | 2836 |
| <I/σ(I)> | 10.7 | 25.9 | 1.8 |
| Completeness [%] | 99.9 | 99.7 | 99.9 |
| Redundancy | 6.5 | 5.6 | 6.6 |
| CC(1/2) | 0.998 | 0.997 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 25% (w/v) PEG 3350, 100 mM Bis-Tris, 200 mM ammonium sulfate |
