6XFJ
Crystal structure of the type III secretion pilotin InvH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-09 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.503, 53.183, 56.851 |
| Unit cell angles | 107.69, 97.20, 95.95 |
Refinement procedure
| Resolution | 44.360 - 1.200 |
| R-factor | 0.1341 |
| Rwork | 0.132 |
| R-free | 0.16800 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.914 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.360 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 82540 | 7937 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 94.5 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.998 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 303 | 80 mM cadmium chloride, 20% PEG 300, 400 mM NaCl, 100 mM sodium acetate |
