6XC1

Crystal structure of bacteriophage T4 spackle and lysozyme in orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979200
Spacegroup name	P 21 21 21
Unit cell lengths	41.084, 46.172, 129.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.490 - 1.920
R-factor	0.1875
Rwork	0.186
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6x6o 1wth
RMSD bond length	0.003
RMSD bond angle	0.561
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.170	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.085	1.800
Number of reflections	19371	1295
<I/σ(I)>	10.9
Completeness [%]	99.3
Redundancy	4.9
CC(1/2)	0.998	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	35% (w/v) polyethylene glycol 3350, 20% 2-propanol, 0.1 M HEPES-NaOH pH 7.5