6XB6
Structure of Danaus plexippus poxin cGAMP nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-10 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.162, 75.414, 77.422 |
| Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
| Resolution | 38.120 - 1.450 |
| R-factor | 0.1578 |
| Rwork | 0.157 |
| R-free | 0.17430 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.120 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rpim | 0.051 | 0.715 |
| Number of reflections | 87244 | 4135 |
| <I/σ(I)> | 7.2 | 1.3 |
| Completeness [%] | 98.6 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.996 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 200 mM ammonium citrate, 25% PEG-3350 |
