6XAX
Structure of a fragment of human fibronectin containing the 11th type III domain, extra domain A, and the 12th type III domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.479, 76.420, 96.796 |
| Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
| Resolution | 39.640 - 2.400 |
| Rwork | 0.252 |
| R-free | 0.31180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dft |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.626 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.640 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 26925 | 2663 |
| <I/σ(I)> | 21.4 | 2.1 |
| Completeness [%] | 95.9 | 95.5 |
| Redundancy | 12.1 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Ca(CH3COO)2, 0.1M imidazole pH 8.0, 10%(w/v) PEG 8000 |
