6XAJ
Crystal structure of NzeB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.826 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.712, 55.808, 56.102 |
| Unit cell angles | 90.00, 101.92, 90.00 |
Refinement procedure
| Resolution | 39.135 - 1.498 |
| R-factor | 0.1898 |
| Rwork | 0.189 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xai |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.837 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.135 | 1.550 |
| High resolution limit [Å] | 1.498 | 1.500 |
| Rmerge | 0.056 | 1.013 |
| Rmeas | 0.061 | 1.097 |
| Number of reflections | 54058 | 5282 |
| <I/σ(I)> | 14.45 | 1.35 |
| Completeness [%] | 99.7 | 97.45 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.999 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 23% PEG 3350, 100 mM DL-malic acid, 2.5% ethylene glycol |
