6XA8

Crystal Structure of Human Scribble PDZ1:Vangl2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-09-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9357
Spacegroup name	F 2 2 2
Unit cell lengths	75.379, 75.379, 212.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.700 - 2.200
R-factor	0.2591
Rwork	0.259
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ms1
RMSD bond length	0.003
RMSD bond angle	0.522
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.703	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.109	0.778
Number of reflections	15662	1349
<I/σ(I)>	8.4	2.3
Completeness [%]	100.0
Redundancy	9.7
CC(1/2)	0.998	0.813

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M Phosphate/citrate pH 4.2 and 40% (v/v) PEG 300