6XA3

Structure of the ligand free P450 monooxygenase TamI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1159
Spacegroup name	I 4 2 2
Unit cell lengths	154.498, 154.498, 91.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.160 - 2.960
R-factor	0.2573
Rwork	0.254
R-free	0.31320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bvj
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.160	3.120
High resolution limit [Å]	2.960	2.960
Rmerge	0.126	0.954
Rmeas	0.139	1.040
Number of reflections	11821	1689
<I/σ(I)>	15	2.9
Completeness [%]	99.7	100
Redundancy	10.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	3.5 M sodium formate, pH 7.0 and 0.1 M strontium chloride