6X42

High Resolution Crystal Structure Analysis of SERA5E from plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-22
Detector	ADSC QUANTUM 210
Wavelength(s)	0.956619
Spacegroup name	H 3
Unit cell lengths	102.288, 102.288, 71.718
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.470 - 1.200
R-factor	0.1268
Rwork	0.126
R-free	0.14660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wbf
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.470	1.243
High resolution limit [Å]	1.200	1.200
Rmerge	0.063
Rmeas	0.066
Rpim	0.020
Number of reflections	87458	8714
<I/σ(I)>	17.72
Completeness [%]	99.9	99.46
Redundancy	11.2	10.6
CC(1/2)	0.999	0.453

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Bis-Tris, NaCl, KH2PO4, PEG-8000, PEG-400