6X3A

Crystal structure of the FN4-FN6 domains of human PTPRD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	I 1 2 1
Unit cell lengths	95.060, 34.570, 109.199
Unit cell angles	90.00, 90.33, 90.00

Refinement procedure

Resolution	34.060 - 1.770
R-factor	0.1975
Rwork	0.195
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Dlar FN5
RMSD bond length	0.006
RMSD bond angle	0.824
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.770	1.770
Rmerge	0.077	0.679
Rmeas	0.088	0.818
Rpim	0.046	0.453
Number of reflections	35136	1959
<I/σ(I)>	13.5	2.4
Completeness [%]	99.5
Redundancy	7.2
CC(1/2)	0.998	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200 mM Magnesium formate, 20% (w/v) PEG 3350