6X1P
Crystal Structure of Choanoflagellate (Monosiga brevicollis) Dlg1 PDZ2 (mbDLG-2) in spacegroup I2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-03-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.977410 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 37.147, 35.259, 63.494 |
Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
Resolution | 34.716 - 1.702 |
R-factor | 0.1849 |
Rwork | 0.182 |
R-free | 0.23810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2byg |
RMSD bond length | 0.006 |
RMSD bond angle | 0.743 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.720 | 1.930 | 1.810 |
High resolution limit [Å] | 1.700 | 1.810 | 1.700 |
Number of reflections | 8933 | 2584 | 2670 |
<I/σ(I)> | 11.39 | 3.88 | 2.23 |
Completeness [%] | 98.4 | 98.2 | 97.3 |
Redundancy | 3.47 | 3.44 | 3.39 |
CC(1/2) | 0.998 | 0.926 | 0.829 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.1 M sodium malonate, pH 5, 12% w/v PEG3350 |