6X0B
Crystal Structure of Thioredoxin NaTrxh from Nicotiana alata
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.990, 68.851, 70.807 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.362 - 1.702 |
R-factor | 0.1871 |
Rwork | 0.185 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iwt |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.362 | 1.700 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.106 | 1.032 |
Rpim | 0.038 | 0.384 |
Number of reflections | 47203 | 2344 |
<I/σ(I)> | 25.2 | 2.6 |
Completeness [%] | 98.7 | |
Redundancy | 8.6 | |
CC(1/2) | 1.000 | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291.15 | 20% (w/v) PEG 3,350, 200 mM ammonium formate and 0.025% (v/v) dichloromethane |