6X05

Nup133 (aa55-481) from S. cerevisiae bound by VHH-SAN4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-17
Detector	PSI PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 2
Unit cell lengths	93.381, 154.693, 41.423
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.570 - 2.100
R-factor	0.1765
Rwork	0.174
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q9t
RMSD bond length	0.008
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.570	2.180
High resolution limit [Å]	2.100	2.100
Rpim	0.052	0.480
Number of reflections	39093	3828
<I/σ(I)>	19.3	1.48
Completeness [%]	99.9
Redundancy	11.9
CC(1/2)	0.999	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	24% PEG 3,350, 0.2M Ammonium sulfate, 0.1M Bis-Tris pH 5.5