6WZX
GID4 in complex with IGLWKS peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.122, 51.190, 55.447 |
| Unit cell angles | 68.23, 89.12, 69.08 |
Refinement procedure
| Resolution | 51.005 - 1.750 |
| Rwork | 0.193 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ccr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.020 | 51.020 | 1.700 |
| High resolution limit [Å] | 1.670 | 8.990 | 1.670 |
| Rmerge | 0.033 | 0.024 | 0.713 |
| Rmeas | 0.044 | 0.032 | 0.967 |
| Rpim | 0.030 | 0.021 | 0.649 |
| Total number of observations | 81854 | 583 | 3638 |
| Number of reflections | 40219 | 276 | 1972 |
| <I/σ(I)> | 11.6 | 36.1 | 0.9 |
| Completeness [%] | 91.4 | 97.2 | 88.5 |
| Redundancy | 2 | 2.1 | 1.8 |
| CC(1/2) | 0.999 | 0.998 | 0.588 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 20% (w/v) PEG 3350 and 8% (v/v) Tacsimate pH 7.0 |
