6WZV

Lon protease proteolytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979460
Spacegroup name	H 3 2
Unit cell lengths	186.276, 186.276, 159.718
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.100 - 2.510
R-factor	0.1786
Rwork	0.177
R-free	0.21390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wys
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.570
High resolution limit [Å]	2.510	6.050	2.510
Rmerge	0.108	0.053	1.388
Rmeas	0.127	0.062	1.650
Rpim	0.066	0.032	0.878
Total number of observations	127739
Number of reflections	35939	2613	2548
<I/σ(I)>	6.8
Completeness [%]	98.9	96.5	99.5
Redundancy	3.6	3.4	3.3
CC(1/2)		0.997	0.349

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	1.6M ammonium sulfate, 0.1M Bicine