6WZR
Fusibacterium ulcerans ZTP riboswitch bound to p-1-pyridinyl AICA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.991, 89.991, 122.834 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.240 - 3.200 |
| R-factor | 0.2103 |
| Rwork | 0.206 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5btp |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.317 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
| Rmerge | 0.083 | 0.042 | 1.503 |
| Rmeas | 0.088 | 0.045 | 1.588 |
| Rpim | 0.029 | 0.016 | 0.511 |
| Total number of observations | 95653 | ||
| Number of reflections | 9937 | 563 | 487 |
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 9.6 | 8.5 | 9.5 |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 26% (w/v) PEG 4000, 100 mM Tris HCl pH 8.5, 180 mM Lithium Sulfate, 4% (v/v) PEG 400 |
