6WZN

Crystal Structure of a Fluorescent Single Chain Fv Chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	103.961, 103.961, 122.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.870 - 2.500
R-factor	0.1671
Rwork	0.163
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tza
RMSD bond length	0.006
RMSD bond angle	0.850
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc1_3777)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.059	1.000
Rpim	0.019	0.467
Number of reflections	24030	1177
<I/σ(I)>	40.3
Completeness [%]	100.0
Redundancy	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	298	1.6M citric acid pH 6.2