6WZM
LY3041658 Fab bound to CXCL8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.012, 103.629, 193.289 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.640 - 2.280 |
R-factor | 0.189 |
Rwork | 0.187 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Internal |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 193.290 | 2.400 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.101 | 0.637 |
Number of reflections | 60358 | 8751 |
<I/σ(I)> | 15.8 | |
Completeness [%] | 99.8 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20% PEG 3350, 200mM Magnesium Chloride |