6WZJ
LY3041658 Fab bound to CXCL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-05 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.042, 200.643, 65.253 |
| Unit cell angles | 90.00, 106.68, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.370 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Internal |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 2.500 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.101 | 0.810 |
| Number of reflections | 55547 | 8122 |
| <I/σ(I)> | 5.9 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 12% PEG 20000, 100mM MES pH 6.5 |
