6WYX
Crystal structure of Pseudomonas 7A Glutaminase-Asparaginase in complex with L-Asp at pH 5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 80.958, 80.958, 176.819 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.060 - 1.480 |
| R-factor | 0.0881 |
| Rwork | 0.088 |
| R-free | 0.11990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pga |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.890 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.100 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.076 | 0.737 |
| Rpim | 0.027 | 0.349 |
| Number of reflections | 216530 | 10676 |
| <I/σ(I)> | 27.4 | 2.2 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 8.3 | 4.9 |
| CC(1/2) | 0.996 | 0.732 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 8% w/v Tacsimate, pH 5.0, 20% w/v PEG3350 |
