6WXQ

Crystal structure of CRISPR-associated transcription factor Csa3 complexed with cA4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2019-11-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92009
Spacegroup name	P 21 21 2
Unit cell lengths	74.793, 118.830, 64.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.640 - 2.050
R-factor	0.1622
Rwork	0.160
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wte
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.092	0.045	0.371
Rmeas	0.098	0.048	0.399
Rpim	0.034	0.016	0.144
Total number of observations	293709
Number of reflections	36695	2017	1810
<I/σ(I)>	9.5
Completeness [%]	100.0	99.8	99.9
Redundancy	8	9.1	7.4
CC(1/2)		0.999	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	0.1 M potassium sulfate, 0.1 M sodium potassium phosphate, pH 5.8, 16% PEG3350