6WXD
SARS-CoV-2 Nsp9 RNA-replicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 88.775, 88.775, 134.042 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.700 - 2.000 |
| Rwork | 0.214 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.012 | 0.404 |
| Number of reflections | 21482 | 1451 |
| <I/σ(I)> | 26.4 | 1.4 |
| Completeness [%] | 99.4 | 93.1 |
| Redundancy | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 2.2M Ammonium Sulfate, 0.1M phosphate-citrate buffer pH 4 |
