6WX7

SOX2 bound to Importin-alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	CCD
Collection date	2012-08-24
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.070, 91.291, 97.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 2.700
R-factor	0.1969
Rwork	0.195
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ul1
RMSD bond length	0.002
RMSD bond angle	0.534
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	18955	2235
<I/σ(I)>	11.2
Completeness [%]	96.4
Redundancy	10.2
CC(1/2)	0.922	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	750mM sodium citrate (pH7), 10 mM DTT