6WWX

Crystal structure of truncated bacteriophage hyaluronan lyase HylP in complex with unsaturated hyaluronan tetra-saccharides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	67.535, 86.819, 168.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.680 - 2.200
R-factor	0.1839
Rwork	0.182
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wv2
RMSD bond length	0.009
RMSD bond angle	1.307
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.081	0.559
Number of reflections	51169	5022
<I/σ(I)>	11.2	4.6
Completeness [%]	99.8	100
Redundancy	8.1	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	10% (v/v) 2-Propanol, 20% (w/v) PEG 4000, 0.1 M Hepes, pH 7.5; incubation of protein HylP with unsaturated Hyaluronan tetra-saccharides at 1:20 molar ratio prior to crystallization