6WW6

Crystal structure of EutV bound to RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-09-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 41 3 2
Unit cell lengths	258.483, 258.483, 258.483
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.190 - 3.800
R-factor	0.2336
Rwork	0.233
R-free	0.24140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wsh
RMSD bond length	0.006
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (dev_3758)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.190	4.250
High resolution limit [Å]	3.800	3.800
Rmerge	0.151	0.799
Rpim	0.084	0.489
Number of reflections	14773	4093
<I/σ(I)>	9.1	2.3
Completeness [%]	99.5	98.9
Redundancy	6.7	6.7
CC(1/2)	0.998	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	0.1 M Sodium chloride 0.1 M HEPES 1.4-1.8 M Ammonium sulfate