Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 70.955, 70.955, 175.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.690 - 1.490 |
| R-factor | 0.1987 |
| Rwork | 0.198 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6OKI |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.897 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.083 | 3.900 |
| Rpim | 0.019 | |
| Number of reflections | 43661 | 2105 |
| <I/σ(I)> | 22.4 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 35.3 | 30.3 |
| CC(1/2) | 1.000 | 0.501 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 20 % w/v PEG 6000, 0.2 M MgCl2, 0.1 M HEPES, pH 7.0 |
