6WUD

Human Calcium and Integrin Binding Protein 3 Bound to TMC1 Residues 303-347

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.77
Spacegroup name	P 32 2 1
Unit cell lengths	100.650, 100.650, 49.723
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	87.170 - 1.840
R-factor	0.1656
Rwork	0.164
R-free	0.18760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wu7
RMSD bond length	0.011
RMSD bond angle	1.088
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.170	1.904
High resolution limit [Å]	1.838	1.838
Number of reflections	23155	1806
<I/σ(I)>	20.93
Completeness [%]	90.8	72.18
Redundancy	17.8
CC(1/2)	0.999	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	25% PEG 3350 0.2M Magnesium Chloride