6WU5
Human Calcium and Integrin Binding Protein 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.46 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.752, 61.752, 180.969 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.320 - 1.880 |
| R-factor | 0.1799 |
| Rwork | 0.178 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xo5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.805 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.18-3845-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.320 | 1.946 |
| High resolution limit [Å] | 1.879 | 1.879 |
| Number of reflections | 33512 | 3270 |
| <I/σ(I)> | 34.59 | |
| Completeness [%] | 99.9 | 99.33 |
| Redundancy | 18.3 | |
| CC(1/2) | 0.999 | 0.901 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.5 M Lithium Sulfate 0.2M Sodium Thiocyanate |
