6WTU

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 273264

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1
Unit cell lengths	93.988, 99.506, 103.487
Unit cell angles	114.72, 104.98, 89.81

Refinement procedure

Resolution	49.031 - 2.550
R-factor	0.2106
Rwork	0.208
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.031	49.031	2.700
High resolution limit [Å]	2.540	7.550	2.540
Rmerge	0.092	0.031	0.729
Rmeas	0.108	0.036	0.858
Total number of observations	380763
Number of reflections	105015	4040	16340
<I/σ(I)>	9.92	32.71	1.67
Completeness [%]	97.5	97.4	94.1
Redundancy	3.626	3.64	3.554
CC(1/2)	0.997	0.998	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	0.1 M sodium cacodylate pH 6.0, 0.1 M magnesium chloride, 15 % (w/v) PEG 4,000