6WTR

Structure of Human pir-miRNA-300 Apical Loop Fused to the YdaO Riboswitch Scaffold

Replaces:  6N5R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.116
Spacegroup name	P 31 2 1
Unit cell lengths	113.070, 113.070, 114.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	74.295 - 3.082
R-factor	0.1678
Rwork	0.165
R-free	0.19220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qk8
RMSD bond length	0.003
RMSD bond angle	0.754
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.295	3.190
High resolution limit [Å]	3.080	3.080
Rmeas	0.092	1.430
Rpim	0.021	0.344
Number of reflections	15789	1297
<I/σ(I)>	18.9	1.7
Completeness [%]	99.2	92.4
Redundancy	18.8	15.3
CC(1/2)	1.000	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4		1.90 M (NH4)2SO4, 0.158 M Li2SO4, and 0.1 M NaHEPES pH 7.4