6WTB

Sort-Tagged Drosophila Cryptochrome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	72.979, 122.186, 81.954
Unit cell angles	90.00, 115.48, 90.00

Refinement procedure

Resolution	39.000 - 2.580
Rwork	0.234
R-free	0.28750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gu5
RMSD bond length	0.002
RMSD bond angle	0.423
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.17.1)
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.620
High resolution limit [Å]	2.580	2.580
Rmerge	0.114	0.604
Rmeas	0.138	0.722
Rpim	0.076	0.391
Number of reflections	39540	3882
<I/σ(I)>	13.3
Completeness [%]	96.6	95.5
Redundancy	3.3	3.2
CC(1/2)	0.985	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	4 microliter drop (2 microliter protein solution, 2 microliter well solution) 100 mM Tris (pH 9) 150 mM Magneisum Acetate Tetrahydrate 17% PEG-4000 8 mg/mL sort-tagged WT Drosophila Cryptochrome