6WT8
Structure of a STING-associated CdnE c-di-GMP synthase from Flavobacteriaceae sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-10-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.181, 78.100, 111.105 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.490 - 1.520 |
| R-factor | 0.1687 |
| Rwork | 0.168 |
| R-free | 0.18800 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.351 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.490 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rpim | 0.031 | 0.701 |
| Number of reflections | 69098 | 3383 |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.7 | |
| Redundancy | 17.6 | |
| CC(1/2) | 0.999 | 0.562 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M sodium acetate pH 4.6, 2 M sodium formate |
