6WSN
Self-assembly of a 3D DNA crystal lattice (4x5 duplex version) containing the J7 immobile Holliday junction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 68.245, 68.245, 61.163 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.122 - 3.052 |
R-factor | 0.2587 |
Rwork | 0.257 |
R-free | 0.28730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kek |
RMSD bond length | 0.011 |
RMSD bond angle | 0.802 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.100 |
High resolution limit [Å] | 3.050 | 8.270 | 3.050 |
Rmerge | 0.082 | 0.069 | 0.975 |
Rmeas | 0.086 | 0.073 | 1.003 |
Rpim | 0.023 | 0.023 | 0.231 |
Number of reflections | 3334 | 191 | 156 |
<I/σ(I)> | 7.5 | ||
Completeness [%] | 99.9 | 97.9 | 100 |
Redundancy | 17.8 | 15.6 | 17.3 |
CC(1/2) | 0.865 | 0.948 | 0.963 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5 mL of 0.05 M Cacodylate pH 7.0, 20 mM MgCl2, 1.0 mM spermine, 1.0 mM CoH18N6, and 15% ethanol was added to the reservoir with 2 uL added to the drop containing 4 uL of DNA stock |