6WSM
Crystal structure of coiled coil region of human septin 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-06 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979499 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 92.007, 92.007, 56.769 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.235 - 2.451 |
| R-factor | 0.3207 |
| Rwork | 0.319 |
| R-free | 0.33890 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.321 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.770 | 56.770 | 2.550 |
| High resolution limit [Å] | 2.450 | 8.830 | 2.450 |
| Rmerge | 0.107 | 0.030 | 1.873 |
| Rmeas | 0.109 | 0.031 | 1.898 |
| Rpim | 0.018 | 0.006 | 0.304 |
| Total number of observations | 207457 | 4461 | 23154 |
| Number of reflections | 5585 | 161 | 601 |
| <I/σ(I)> | 15 | 62.4 | 1.6 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 37.1 | 27.7 | 38.5 |
| CC(1/2) | 1.000 | 1.000 | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 291 | 100mM Citric acid, 1.25M Ammonium Sulphate |
