6WSK
Crystal Structure of the Cannabinoid Receptor 1 Interacting Protein 1a (CRIP1a)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-01 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.177, 68.177, 146.648 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.860 - 1.550 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fa0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.097 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX (1.14_3260) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.860 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.059 | 1.250 |
| Rmeas | 0.060 | 1.310 |
| Rpim | 0.010 | 0.370 |
| Number of reflections | 50935 | 4957 |
| <I/σ(I)> | 28.4 | 2 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 20.1 | 11.6 |
| CC(1/2) | 1.000 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30 mg/mL, 0.1 M sodium citrate (pH 4-5.5), and 0.3-0.6 ammonium sulfate |
