6WSH

Crystal structure of EutV from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	54.539, 85.688, 97.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.840 - 2.120
R-factor	0.2392
Rwork	0.239
R-free	0.24050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s8n 1qo0
RMSD bond length	0.008
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (dev_3758)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.850	2.180
High resolution limit [Å]	2.120	2.120
Number of reflections	26642	2134
<I/σ(I)>	6
Completeness [%]	99.3	100
Redundancy	4.9	2.9
CC(1/2)	0.984	0.377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	0.075M Tris pH 8.5, 18-20% v/v tert-butanol, 25% v/v glycerol