6WQU
CSL (RBPJ) bound to Notch3 RAM and DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.450, 97.660, 104.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.390 - 2.410 |
| R-factor | 0.2005 |
| Rwork | 0.199 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iag |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.660 | 97.660 | 2.500 |
| High resolution limit [Å] | 2.410 | 9.020 | 2.410 |
| Rmerge | 0.109 | 0.067 | 0.583 |
| Rmeas | 0.121 | 0.075 | 0.659 |
| Rpim | 0.050 | 0.033 | 0.299 |
| Total number of observations | 147091 | 3040 | 12050 |
| Number of reflections | 26714 | 591 | 2712 |
| <I/σ(I)> | 9.7 | 17.5 | 2.7 |
| Completeness [%] | 99.4 | 98.2 | 97.7 |
| Redundancy | 5.5 | 5.1 | 4.4 |
| CC(1/2) | 0.994 | 0.993 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8 | 277.15 | 0.2 M ammonium fluoride, 14% PEG 3350 |
