6WQO

Plasmodium vivax reticulocyte binding protein 2b (PvRBP2b) bound to human monoclonal antibody 283284

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	88.250, 126.177, 149.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.323 - 3.150
R-factor	0.2567
Rwork	0.254
R-free	0.30480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w53
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.546	48.546	3.330
High resolution limit [Å]	3.140	9.270	3.140
Rmerge	0.260	0.074	1.113
Rmeas	0.288	0.082	1.223
Total number of observations	161509
Number of reflections	29437	1263	4503
<I/σ(I)>	6	18.06	1.42
Completeness [%]	98.7	98.3	95.2
Redundancy	5.487	5.257	5.632
CC(1/2)	0.981	0.995	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	0.3 M ammonium nitrate, 0.1 M sodium HEPES pH 7.0, 26% (w/v) PEG 2,000