6WQI

Xanthomonas citri Methionyl-tRNA synthetase (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.4586
Spacegroup name	P 1 21 1
Unit cell lengths	74.017, 95.253, 84.080
Unit cell angles	90.00, 90.52, 90.00

Refinement procedure

Resolution	47.830 - 2.000
R-factor	0.1874
Rwork	0.185
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wq6
RMSD bond length	0.015
RMSD bond angle	1.657
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.830	2.040
High resolution limit [Å]	2.000	2.000
Rmerge	0.107	0.918
Rmeas	0.127	1.107
Rpim	0.069	0.609
Number of reflections	76238	4423
<I/σ(I)>	8.6
Completeness [%]	97.0	95
Redundancy	3.3	3.1
CC(1/2)	0.996	0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Tris-HCl, 28 - 32% PEG4000, 0.2 M MgCl2, 0.45 % Polyvinylpyrrolidone K15