6WQ1

Eukaryotic LanCL2 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	52.415, 108.012, 152.013
Unit cell angles	90.00, 91.26, 90.00

Refinement procedure

Resolution	37.608 - 2.295
R-factor	0.1868
Rwork	0.184
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6u
RMSD bond length	0.008
RMSD bond angle	0.904
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.295	2.295
Rmerge	0.104	0.283
Rpim	0.046	0.166
Number of reflections	71159	2209
<I/σ(I)>	13.9
Completeness [%]	94.0
Redundancy	5.6
CC(1/2)	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273	PEG400, PEG4000