6WPZ
The structure of Pf4r from a superinfective isolate of the filamentous phage Pf4 of Pseudomonas aeruginosa PA01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.444, 45.128, 168.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.353 - 1.993 |
| R-factor | 0.1903 |
| Rwork | 0.186 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6wnm |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.655 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.930 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.195 | 1.052 |
| Number of reflections | 9885 | 1417 |
| <I/σ(I)> | 4.6 | 1 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.5 | 3.2 |
| CC(1/2) | 0.990 | 0.513 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Polyethylene glycol monomethyl ether 550 25% (v/v) 50 mM HEPES; pH 7.0 10 mM Magnesium chloride Protein at 50 mg/ml. Frozen in cryo protectant 20 % PEG200 |
