6WPQ

GNYNVF from hnRNPA2-low complexity domain segment, residues 286-291, D290V variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	4.780, 19.000, 20.740
Unit cell angles	90.00, 95.71, 90.00

Refinement procedure

Resolution	20.640 - 1.100
R-factor	0.0772
Rwork	0.074
R-free	0.10380
Structure solution method	AB INITIO PHASING
RMSD bond length	0.015
RMSD bond angle	1.822
Data reduction software	XDS
Data scaling software	XSCALE (VERSION Jan 26, 2018 BUILT=20180808)
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.640	20.640	1.150
High resolution limit [Å]	1.100	3.980	1.100
Rmerge	0.106	0.087	0.185
Rmeas	0.116	0.095	0.218
Total number of observations	7420
Number of reflections	1329	37	75
<I/σ(I)>	10.45	14.18	5.67
Completeness [%]	86.5	97.4	41.7
Redundancy	5.583	6.216	3.467
CC(1/2)	0.992	0.995	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	salt: 0.15M Ammonium Acetate, precipitant: 35% MPD, buffer: 0.1M Bis-Tris