6WPG

Structural Basis of Salicylic Acid Perception by Arabidopsis NPR Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-11-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 31 2 1
Unit cell lengths	88.293, 88.293, 138.003
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.418 - 2.283
R-factor	0.2061
Rwork	0.205
R-free	0.22380
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.370
High resolution limit [Å]	2.283	4.930	2.290
Rmerge	0.064	0.033	0.771
Rmeas	0.067	0.035	0.820
Rpim	0.020	0.010	0.274
Number of reflections	28861	3058	2828
<I/σ(I)>	17.8
Completeness [%]	100.0	99.6	100
Redundancy	10.6	11.1	8.4
CC(1/2)		1.000	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.25 M potassium phosphate dibasic, 23% PEG 3350, pH 9.2