6WOY
Thermus thermophilus RNA polymerase initially transcribing complex with 3'dCTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 185.977, 102.465, 296.043 |
Unit cell angles | 90.00, 98.86, 90.00 |
Refinement procedure
Resolution | 29.980 - 3.000 |
R-factor | 0.2061 |
Rwork | 0.206 |
R-free | 0.21900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4q4z |
RMSD bond length | 0.009 |
RMSD bond angle | 1.119 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rpim | 0.054 | 0.358 |
Number of reflections | 106804 | 4013 |
<I/σ(I)> | 14.78 | 1.73 |
Completeness [%] | 96.9 | 73.6 |
Redundancy | 3.4 | 2.9 |
CC(1/2) | 0.855 | 0.855 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 0.1 M Tris-HCl (pH 8), 0.2M KCl, 50 mM MgCl2, 10% PEG4000 |