6WOX
Thermus thermophilus RNA polymerase initially transcribing complex with 2'dCTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.435, 102.654, 295.382 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 29.930 - 3.140 |
| R-factor | 0.1971 |
| Rwork | 0.196 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q4z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.223 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.140 | 3.150 |
| Rpim | 0.085 | 0.370 |
| Number of reflections | 89931 | 3279 |
| <I/σ(I)> | 10.06 | 1.44 |
| Completeness [%] | 94.3 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.829 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 0.1M Tris-HCl (pH 8), 0.2M KCl, 50 mM MgCl2, 10% PEG4000 |
