6WOP
Crystal structure of gamma-aminobutyrate aminotransferase PuuE from Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-08-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 92.379, 188.453, 114.113 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.850 |
| R-factor | 0.1819 |
| Rwork | 0.181 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sff |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.413 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15_3448) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.112 | 0.384 |
| Rpim | 0.042 | 0.272 |
| Number of reflections | 84930 | 4194 |
| <I/σ(I)> | 20.5 | 2.05 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | |
| CC(1/2) | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% (w/v) PEG3350, 0.2M potassium sodium tartrate, 5 mM Glutamic acid |
