6WO6
Structure of RavA (lpp0008) E38A/K39A surface entropy reduction mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-18 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 51.460, 62.670, 286.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.820 - 2.800 |
| R-factor | 0.2039 |
| Rwork | 0.203 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.535 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.820 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 22566 | 1679 |
| <I/σ(I)> | 9.96 | |
| Completeness [%] | 94.5 | 95.4 |
| Redundancy | 3.7 | 3.82 |
| CC(1/2) | 0.997 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Ammonium citrate tribasic pH 7.0, 20% w/v Polyethylene glycol 8000 and 15% glycerol |
