6WO4

Structure of Hepatitis C Virus Envelope Glycoprotein E2 core from genotype 6a bound to broadly neutralizing antibody HC11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	60.706, 67.111, 222.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.368 - 2.349
R-factor	0.2102
Rwork	0.209
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wo3
RMSD bond length	0.004
RMSD bond angle	0.643
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.390
High resolution limit [Å]	2.349	2.349
Number of reflections	36627	1693
<I/σ(I)>	25.7
Completeness [%]	94.5
Redundancy	9.1
CC(1/2)	0.920	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.16 M ammonium sulfate, 20% (w/v) PEG 4000, 20% (v/v) glycerol, 0.08 M sodium acetate, pH 4.6